Posts Tagged ‘barcode’

Notes from the international barcode of life conference #bol4

// December 5th, 2011 // 1 Comment » // Science Communication

Image by .jun, flickr

On Friday I went to day three of the international barcode of life conference, which happened to be in my hometown of Adelaide, actually at the home of my undergrad – The University of Adelaide – how convenient!

DNA barcoding matches a region of DNA to a species, at the moment there’s still plenty of work on building up that barcode database (called BOLD, though GenBank is also used). There are about a million and a half barcodes recorded so far and it’s streaming along.

The database is open access, and people can use it to match a barcode region from an unknown sample to a species.

So far, people have used this to check out the slice of fish in sushi, illegally collected shark fins, and plenty of other stuff.

It’s a powerful technique now in it’s ninth year and with some serious momentum behind it. There were 450-ish delegates at the conference from around the world, and Australia is a fair trek for most of them.

There’s talk that one day DNA sequencing will be so fast and cheap, you could take a sample while walking through the woods and be linked to species information on a handheld device – you would know if it was poisonous, endangered, new to science or what. Still a while away, but sci-fi in its possibilities.

This cool video gives a neat overview. It’s about a project proposal for student/citizen science in barcoding which is unfortunately currently unfunded and basically on ice at the moment. Nonetheless it’s a cute cartoons and great summary.

The region used for barcoding is called CO1 (found in mitochondira) in animals. It’s x base pairs long, and is generally very different between species, but pretty similar within one species. It’s short enough that sequencing is cheap and quick. A different region is used for fungi (called ITS, which was announced as the official fungi barcode at the conference), and plants use two regions, rbcL and matK, (found in chloroplasts).

The session I went to was on education and engagement – how to get people involved in DNA barcoding.

I love open access, power to the people, breaking down barriers stuff, and they’ve got some sweet plans. Already some projects have been successful, like the urban barcode project that gets high school students involved, and one group, who found the ingredients of tea didn’t always match what’s on the label, were even published in a journal (No less than Nature Scientific Reports! Amazing!) One group found a new species of cockroach, which is like my least favourite insect, but still a good effort.

What's in your tea? Image by massdistraction, flickr

BOLD are in the process of adding education and engagement to their online database so students can add to the database and store their results in a quarantined area. So they have a safe space to experiment with barcoding. Plus then they don’t screw it all up, right? Karen James, who moderated the session, actually pointed out that students may be less likely to make mistakes, as they are only working with a small number of samples and there’s less chance of losing track and accidental mislabeling.

Still in development, the BOLD 3.0 interface will look less intimidating than the current version, making it clearer for n00bs like me, and with links for educators at the bottom. They’re beta version is online here. Neat. I played around with BOLD before, taking a look at the barcode regions out of curiousity, and with my amateur skillz found it a bit tricky to navigate. Can’t wait to see the new one up and running so I can play with it.

If you want to read more about DNA barcoding, I recommend the iBOL website. I’ve got some more bits and pieces, but will post them separately once I’ve had a chance to flesh them out properly.

Inspiring Australia and the barcode of life, conferences

// February 7th, 2011 // Comments Off on Inspiring Australia and the barcode of life, conferences // Science Communication

Couple of interesting conferences coming up this year. The first one is Inspiring Australia in Melbourne from March 28-29. ‘Tis a science communication conference, tackling topics like social media, politics, and a whole bunch of exciting sci-comm stuff. Registration opens today, and it will be good.

The second conference is the Consortium for the Barcode of Life which will be hosted in bonny old Adelaide in November this year. Adelaide Uni scored the gig after competition with 19 applicants from around the globe. Previously the event has been hosted in Mexico City, Taipai and London.

DNA code is a bit like a barcode to begin with: Information hidden in a mysterious pattern that only a computer can analyse. The barcode of life refers to specifically to a certain small section of DNA which can be used to compare species. The section changes between species, but stays the same within members of the same species, and is accurate for most mammals and bees.

The consortium is to discuss and co-ordinate how to take DNA samples from all the animals IN THE WORLD, and have them on a giant database. Then when future scientists find a weird animal, they can take a sample, scan it in and *beep*, one lemur for $9.99. It’s a neat idea, and totally exciting that Adelaide is hosting this International event.






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